Comparing Castings and Forgings
Many cell types will grow when attached to a rigid surface but not in suspension, a phenomenon termed ?
[url=http://www.nb-xingfeng.com/anchorage/]anchorage[/url] dependence”?. Anchorage dependence can be studied by
incorporating solid particles of varying size into gels. It has been found that colonies will form on glass fibrils 500 μ in
length, but not in the presence of silica fragments smaller than the cells. This shows that the suspending medium is not
itself inhibitory, and confirms the requirement for a rigid surface of adequate size.
The state of inhibited cells in suspension culture was examined by dispersing them in a methyl cellulose gel, in vessels
lined with agar. In this sys